Development of Optimized Ex Vivo Organotypic Slice Culture Systems

The main goal of this project is to develop an efficient and robust ex vivo tumor slice cultures to bridge the gap between in vitro cell culture studies and in vivo animal model studies. The current mainstream pipeline for drug discovery heavily relies on initial studies in established human cancer cell lines; however, these cell lines lack the cellular complexity present in most malignant tumors and have diverged from the original tumor at the molecular level; hence, no longer modeling the original disease in the patient.

While in vivo animal models provide the organismal complexity for the most part, animal studies are less amenable to experimental manipulations, very costly, and time consuming. In contrast, organotypic slice cultures (live tissues cut to thin slices) are amenable to genetic, chemical and physiological manipulations that can be monitored in real time, over long periods of time, and in longitudinal studies. Furthermore, they can provide relatively cost effective and rapid experimental paradigms. In addition, they provide platforms to study native cellular interactions between cancer cells and their microenvironment. By combining genetic tools that are available, such as mouse strains that have unique populations of cells labeled with different fluorescent molecules, tumor slice cultures can advance our understanding of cancer biology to a new level, improve drug development pipelines, and even inform smarter therapy combinations. Therefore, we propose to develop, optimize, and validate tumor slice cultures from brain, breast, colon and pancreatic cancers in this study.

Organization: 
The Jackson Laboratory
Researcher: 
Kyuson Yun, Ph.D
Grant Amount Given: 
$164,686
Year Issued: 
2013
Period: 
Annual
Grant Category: 
Research
Types of Cancer: 
Brain
Breast
Colon
Other
Pancreatic
Grant Duration: 
2 Year Accelerator Grant

Maine Cancer Foundation Grants to this Organization:

Year Program Amount Category Organization
2014 A Slice of Life: The Search for Variation in Brain Tumors $50,000 Research The Jackson Laboratory
2014 Genetic Testing for Primary Care Providers: Case-based Web Modules $49,220 Education The Jackson Laboratory
2014 A Secondary Syndrome: Acute Myeloid Leukemia $50,000 Research The Jackson Laboratory
2014 Are Micro RNAs the Key to Stopping Lung Cancer? $50,000 Research The Jackson Laboratory
2014 Mouse Models for Acute Myeloid Leukemia $169,834 Research The Jackson Laboratory
2013 Development of Optimized Ex Vivo Organotypic Slice Culture Systems $164,686 Research The Jackson Laboratory
2013 Defining Susceptibility To Transformation By Epigenetic Landscape $49,999 Research The Jackson Laboratory
2013 Assessing Therapeutic Potential of a New Drug ICG-001 in Glioblastoma $50,000 Research The Jackson Laboratory
2013 The Maine Triple-Negative Breast Cancer Study $212,601 Research The Jackson Laboratory
2012 FoxC2 Function in glioma stem cells and Vasculature $84,000 Research The Jackson Laboratory
2012 New Models for Lung Cancer Research $199,036 Research The Jackson Laboratory
2012 Online CME Course $27,600 Education The Jackson Laboratory
2011 Imaging Glicoma Stem Cell Dynamics in Vivo $90,000 Research The Jackson Laboratory
2010 MicroRNA Expression during Lung Tumor Progression $90,000 Research The Jackson Laboratory
2010 Genetic Chemotherapy: Inducing Leukemia and Lymphoma Cell Self Destruction $67,500 Research The Jackson Laboratory
2010 Notch Signaling in Medulloblastoma Initiation and Cancer Stem Cell Maintenance $90,000 Research The Jackson Laboratory
2009 Validating S100A6 as a Novel Marker and Potential Therapeutic Target of Brain Cancer Stem Cells $80,000 Research The Jackson Laboratory
2008 Development Genomics of Lung Cancer $79,524 Research The Jackson Laboratory
2008 Homologous Recombination Factor XRCC2 in B-Cell Genome Stability and Tumor Supression $79,696 Research The Jackson Laboratory
2006 The Role of Src Kinases in Regulation of Stem Cell Survival in CML $75,000 Research The Jackson Laboratory
2006 Chromatin Reorganization in Pro-B Cell Lymphoma Nuclei $74,180 Research The Jackson Laboratory
2005 A Gene Expression Study for the Hormone Independent Growth Transition of Ovarian Cancer Cells in vivo $20,000 Research The Jackson Laboratory
2004 Malignant Ovarian Granulose Cell Teumorigenesis $37,165 Research The Jackson Laboratory
2003 Parent-of-Origin Genetics in a Model of Ovarian Ganulosa Cell Tumorigenesis $35,500 Research The Jackson Laboratory