Cytometric Profiling Of The Non-Malignant Leukocytes In Patients With CLL: Addition Of A Control Group

Flow cytometry is a standard method for characterizing human immune system defects and hematologic malignancies. In such analysis, samples are characterized by the relative abundance of characteristic cell types, which are identified by the presence or absence of specific fluorescently labeled cell-surface proteins. Standard flow analysis is performed through visualization of the data in one- or two-dimensional plots, manually scoring the abundance of known critical cells.

However, the measurements are typically collected in pools of up to eight distinct fluorochromes, potentially facilitating significantly more detailed analysis and refined sample characterization. We propose here to develop novel automated computational methods for the analysis of multidimensional flow cytometry data from peripheral blood specimens of patients with chronic lymphocytic leukemia (Cll). We expect that this mUlti-dimensional characterization will reveal detailed patterns of relative populations of immune cells, resulting in better patient characterization, potentially leading to improved diagnosis and treatment regimens for patients with Cll.

Organization: 
Eastern Maine Medical Center Cancer Care
Researcher: 
Jens Rueter, MD
Grant Amount Given: 
$49,900
Year Issued: 
2013
Period: 
Annual
Grant Category: 
Research
Types of Cancer: 
Cellular
Leukemia
Grant Duration: 
1 Year Pilot Grant

Maine Cancer Foundation Grants to this Organization:

Year Program Amount Category Organization
2013 Cytometric Profiling Of The Non-Malignant Leukocytes In Patients With CLL: Addition Of A Control Group $49,900 Research Eastern Maine Medical Center Cancer Care